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PCR-Based Molecular Breeding Marker Systems

We use an assortment of different markers in MAS breeding programs to analyze seedlings quickly with genetic markers linked to highly desirable agronomic traits. 

Marker-Assisted Selection (MAS)

Through MAS, we identify the data from early generation DNA testing and pertinent phenotypic information to select individuals with desirable propagation traits for subsequent generations. Trait selection maximization is vital for accurate product development, and for reducing the time-to-market for new varieties.

DNA Marker-Assisted Backcrossing (MAB)

We apply MAB to hasten gene introgression and the recovery of the recipient genome in backcrossing experiments. MAB minimizes the linkage drag around the desired gene, and maximizes the chances of identifying the best individuals as parentals for the next generation of a genetic improvement program.

DNA Fingerprinting and Intellectual Rights Protection

The range and types of molecular-based DNA markers are limitless and thus far superior to traditional morphological and isozyme markers for plant variety identification. DNA markers can produce higher levels of variations and polymorphisms and are not likely to be subject to environmental influences.

Custom DNA Marker Development (DMD)

Ag-Biotech offers custom DMD for identifying and developing linked markers for customized trait mapping. Developing molecular markers enables selective breeding of agronomic species. DMD also supports linkage mapping and MAS breeding strategies, which hasten the transfer and introgression of desirable genes among varieties and from wild species into crop plants.

Hybrid- and Trait-Purity Analysis

We determine true hybrids from selfs, identify off types and insure the highest seed quality. Ag-Biotech offers highly accurate and undisputable PCR-based DNA methodologies and reliable DNA-based markers to determine percent hybrid purity and variety identification for various crops.

Genetic Map Construction

We construct gene or linkage maps using molecular markers to mark the locations and relative positions of specific genes on nuclear chromosomes. Using linkage analysis to determine gene loci distances, we identify genes that may be associated with the expression of agronomically important traits, then tag and trace these genes with molecular markers as MAS for their presence or absence.